2nd Programmable Nucleases Course in April – registration open
2nd Programmable nucleases (CRISPR/Cas9) Transgenesis Course at CCP
This course, jointly provided by the Czech Centre for Phenogenomics and PHENOMIN-ICS from Strasbourg, both members of INFRAFRONTIER, is designed to give practical, hands-on training in current technologies and methods employing CRISPR/Cas9-mediated genome manipulations in the generation of mice mutants. Participants will gain theoretical and practical knowledge from our expert instructors, have ample opportunity for discussion and can bring their own targeting projects to work on during the bioinformatics session. The course has been designed for participants with an intermediate knowledge of molecular biology.
Course highlights:
• Design targeting strategy for your project of interest
• Apply the targeting tools and validate CRISPR activity
• Deliver the editing tools to cell lines and zygotes
• Identify CRISPR induced genomic modifications
Date: April 3-7, 2017
Place: Czech Centre for Phenogenomis, Vestec, Czech Republic
Application deadline: 15th February 2017 (results posted 20th February 2017)
Participants: A maximum of 15 participants including 2 scholarships.
Course fee: 980 € for participants from academic institutions and universities. The cost for participants from industry is available on request.
Participation on the course is subject to a selection process. Applications must be accompanied by a short CV, letter of motivation (max 200 words), and a short letter of support. Accommodation and transport costs are not included in the fee and has to be covered by participants on their own. Lunches and refreshments during the course as well as joint dinners are included.
Participants wishing to apply for the scholarship should state this clearly in the cover letter. Please note that the scholarship will cover the course fee only.
Applications should be sent to the following email address: ccp-tam@img.cas.cz
The lecturers and instructor team
The participants of the 2nd programmable nucleases (CRISPR/Cas9) Transgenesis Course will be trained by the experienced team of people who are also equipped with the experience of organizing the “hands-on nuclease course’ accompanying the Transgenic technology meeting TT2016.
Guest lecturers:
Lluis Montoliu (CNB, Spain)
Bernd Zetsche (Broad Institute, USA)
Ronald Naumann (MPI-CBG, Dresden)
Czech Centre for Phenogenomics (IMG/BIOCEV)
Radislav Sedlacek
Björn Schuster
Inken M Beck
Jana Kopkanova
and all other members of Transgenic and Archiving Module of CCP
PHENOMIN-ICS, Strasbourg
Yann Herault
Marie-Christine Birling
Guillaume Pavlovic
Preliminary program (Updated PDF file)
Day 1 – Monday 3rd April 2017
10:45 – 11:00 Arrival and Registration
11:00 – 11:15 Welcome and Introduction to the course and teaching material
Introduction of team members
Radislav Sedlacek (IMG/CCP)
11:15 – 13:15 Participants: presentation individual projects
Radislav Sedlacek (IMG/CCP) and all instructors
13:15 – 14:15 Lunch
14:15 – 14:45 Lecture: Pros-and-cons of programmable nucleases
Radislav Sedlacek (IMG/CCP)
14:45 – 16:15 Practical training session: gRNA validation I
Jana Kopkanová & Björn Schuster (IMG/CCP)
16:15 –17:00 Lecture: Historical perspective – how we came to nucleases
Lluis Montoliu (CNB, Spain)
17:00 –18:00 get together with time for discussions with the speaker and instructors
19:00 Course Dinner 1
Day 2 – Tuesday 4th April 2017
08:00 – 08:45 Lecture: Breaking-Cas, a new guide designing program and CRISPR/Cas function
Lluis Montoliu (CNB, Spain)
08:00 – 08:15 Daily agenda and outcomes overview
09:00 – 10:00 Practical training session: gRNA preparation I
Jana Kopkanová & Björn Schuster (IMG/CCP)
10:00 – 10:15 Tea and Coffee
10:15 – 13:15 Practical training sessions:
- Zygote injection – Ronald Naumann (MPI-CBG, Dresden) and Inken Beck (IMG/CCP)
- Genotyping, digital PCR – Jana Kopkanova (IMG/CCP) and Guillaume Pavlovic (PHENOMIN-ICS)
- Bioinformatics, gene targeting and CRISPR design – Björn Schuster (IMG/CCP), Marie-Christine Birling (PHENOMIN-ICS), Lluis Montoliu (CNB, Spain), Bernd Zetsche (Broad Institute, USA)
13:15 – 14:15 Lunch
14:15 –14:45 Lecture: gRNA validation and genotyping of Indel mutations
Björn Schuster (IMG/CCP)
14:45 – 16:15 Practical training session: gRNA preparation II
Jana Kopkanová & Björn Schuster (IMG/CCP)
16:15 – 17:00 Lecture: CRISPR technology in cell culture
Speaker: tba (Sigma)
17:00 –18:00 get together with time for discussions with the speaker and instructors
18:00 End of Day
Day 3 – Wednesday 5th April 2017
08:00 – 08:45 Lecture: repertoire of gene targeting methods in rodents
Guillaume Pavlovic (PHENOMIN-ICS), Marie-Christine Birling (PHENOMIN-ICS)
08:45 – 09:00 Agenda for the day with tea and coffee
09:00 – 10:00 Practical training session: gRNA preparation III
Jana Kopkanová & Björn Schuster (IMG/CCP)
10:00 – 10:15 Tea and Coffee
10:15 – 13:15 Practical training sessions:
- Zygote injection – Ronald Naumann (MPI-CBG, Dresden) and Inken Beck (IMG/CCP)
- Genotyping, digital PCR – Jana Kopkanova (IMG/CCP) and Guillaume Pavlovic (PHENOMIN-ICS)
- Bioinformatics, gene targeting and CRISPR design – Björn Schuster (IMG/CCP), Marie-Christine Birling (PHENOMIN-ICS), Lluis Montoliu (CNB, Spain), Bernd Zetsche (Broad Institute, USA)
13:15 – 14:15 Lunch
14:15 –14:45 Lecture: Targeting vector design
Björn Schuster (IMG/CCP)
14:45 – 16:15 Practical training session: gRNA validation II
Jana Kopkanová & Björn Schuster (IMG/CCP)
16:15 – 17:00 Lecture: Expanding the Research Applications for CRISPR
Guillaume Pavlovic (PHENOMIN-ICS)
17:00 –18:00 get together with time for discussions with the speaker and instructors
18:00 End of Day
Day 4 – Thursday 6th April 2017
08:00 – 08:45 Lecture: Lecture: Development CRISPR-Cas9 & Cpf1 technology
Bernd Zetsche (Broad Institute, USA)
08:45 – 09:00 Agenda for the day with tea and coffee
09:00 – 10:00 Practical training session: gRNA validation III
Jana Kopkanová & Björn Schuster (IMG/CCP)
10:00 – 10:15 TEA AND COFFEE
10:15 – 13:15 Practical training sessions:
- Zygote injection – Ronald Naumann (MPI-CBG, Dresden) and Inken Beck (IMG/CCP)
- Genotyping, digital PCR – Jana Kopkanova (IMG/CCP) and Guillaume Pavlovic (PHENOMIN-ICS)
- Bioinformatics, gene targeting and CRISPR design – Björn Schuster (IMG/CCP), Marie-Christine Birling (PHENOMIN-ICS), Lluis Montoliu (CNB, Spain), Bernd Zetsche (Broad Institute, USA)
13:15 – 14:15 LUNCH
14:15 –14:45 Lecture: CRISPR/nuclease troubleshooting and genotyping
Bjorn Schuster and Jana Kopkanova (IMG/CCP)
14:45 – 16:15 Practical training session: collection of results and time for discussion/ trouble shooting
All instructors
16:15 – 17:00 Lecture: genome editing using nucleases: new possibilities and application to reveal gene function in diseases
Marie-Christine Birling (PHENOMIN-ICS)
17:00 –18:00 get together with time for discussions with the speaker and instructors
19:00 Course Dinner 2
Day 5 – Friday 7th April 2017
08:00 – 08:45 Lecture: “from genotype to phenotype”
Yann Herault (IBGMC, Strasbourg) or invited speaker
08:45 – 09:00 Agenda for the day with tea and coffee
09:00 – 10:00 Collection of results and preparation of short presentations
All instructors
10:00 – 10:15 Tea and Coffee
10:15 – 12:30 Short group presentations
Summary & Conclusions
12:30 – 13:15 LUNCH
13:15 End of course
Optional:
13:15 – 15:00 Tour through Czech Centre for Phenogenomics (phenotyping and breeding)