• ln vivo body imaging – microCT, luminescence, fluorescence, ultrasound including Echo and 4D, endoscopy

• High resolution ex vivo imaging (embryos, mineralization of bones, assessment of villi in intestine etc.)

• In vivo MRI body composition analysis – quantification of body fat-muscle-liquid tissue applicable for metabolism tests and disease severity monitoring

• Ultrasound comprehensive imaging – 2D&3D, including high resolution probe for mouse embryos. Heart functions measurements with or without challenge (blood pressure, EKV, Power Doppler, Treadmill) – detailed analysis, vascularization of tumors

• Lung functions measurement, histological analysis and flow cytometry (FCM) of bronchoalveolar lavage

• Other in vivo comprehensive measurements and special applications: Eye defects – eye morphology and function, intra-ocular or other ultrasound driven injections; ERG; Auditory brain response test; Behavioral tests – open field and various mazes, gait analysis, grip strength, rotarod, intellicages etc.; Calorimetry measurements including circadian rhythms, glucose metabolism tests

• Blood analysis – flow cytometry (standardized and customized panels), clinical biochemistry & other blood plasma markers (e.g. cytokine multiplex, hormones), hematology including coagulometry

• Flow cytometry assessment of body liquids, tissues and tumor microenvironment (including advanced immunological analysis, T cells in vitro activation)

• Detailed histopathology – standard organ macroscopical and microscopical pathology, including special stains, immunohistochemistry, model specific markers, slide scans for later evaluation, customized design

• MALDI imaging of cells or tissues

• Mass spectrometry, HPLC and other analytical methods for drug or metabolites assessment – established panels: Steroid hormones, bile acids, amino acids, bio-active amines, lipidomics, drug metabolites, other metabolites (list on demand – approx. 200 molecules)

GLP certified services:

• Terminal mice blood sampling under isofluran anesthesy
• Mice euthanasia
• Complete blood count and differential
• Clinical biochemistry measurement on automated analyzer
• Statistical evaluation of data

Other available tests of the unit are also provided according to highly standardized protocols in GLP-like mode.

GLP certified services:

• Complete mice necropsy including gross pathology assessment and organ collection for further processing
• Complete tissue processing from appropriate fixation to staining; stainings currently available under GLP:
  • Hematoxylin & Eosin
  • Masson Trichrome
  • PAS staining
  • Oil red staining
  • Sirius red
  • NASDCL staining
  • Perls’ staining of iron ions
• Evaluation of tissue pathology
• Slide scanning
• Statistical evaluation of data

Other available tests of the unit are also provided according to highly standardized protocols in GLP-like mode.

---