Spink5 LOF (Lost of Function)

Conditional Spink5 knockout was achieved through the insertion of exon 5 (Spink5) surrounded by loxP sites into the chromosome 18. Cre recombination creates loss of exon 5 and it leads to the frameshift. With Krt14creert2 driver the Spink5 ablation can be directed to skin or with R26 creert2 can cause systemic gene ablation. The induction by tamoxifen scheme can be either sytemic or local.